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Pascale, R.* ; Cataldi, T.R.I.* ; Bianco, G.* ; Schmitt-Kopplin, P. ; Bosco, F.* ; Vignola, L.* ; Uhl, J. ; Lucio, M. ; Milella, L.*

Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.).

Food Chem. 242, 497-504 (2017)
Postprint DOI Order publishers version
Open Access Green
The present study deals with the evaluation of antidiabetic activities of Fagioli di Sarconi beans (Phaseolus vulgaris), including 21 ecotypes protected by the European Union with the mark PGI (i.e., Protected Geographical Indication), and cultivated in Basilicata (southern Italy). For this purpose, α-glucosidase and α-amylase assays were assessed; among all bean ecotypes, the tight green seed colour of Verdolino extracts exhibited the highest α-glucosidase and α-amylase inhibitory activity with IC50 = 1.1 ± 0.1 μg/ml and IC50 = 19.3 ± 1.1 μg/ml, respectively. Phytochemical compound screening of all Fagioli di Sarconi beans performed by flow injection-electrospray ionization-ultrahigh resolution mass spectrometry (uHRMS) and based on the calculation of elemental formulas from accurate m/z values, was helpful to annotate specific compounds, such as alkaloids, saponins, flavonoids, and terpenoids, which are most likely responsible for their biological activity.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Fagioli di Sarconi beans; Phaseouls vulgaris L.; High-resolution mass spectrometry; Phytochemical profile; Antidiabetic activity; α-Glucosidase; α-Amylase; Type-2 Diabetes-mellitus; Alpha-amylase; Organic-matter; Glucosidase; Legumes; Antioxidant; Inhibitors; Pathways; Saponins; Acarbose
ISSN (print) / ISBN 0308-8146
e-ISSN 1873-7072
Journal Food Chemistry
Quellenangaben Volume: 242, Issue: , Pages: 497-504 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam [u.a.] ; Jena
Reviewing status Peer reviewed