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Schmitz, F. ; Burtscher, I. ; Stauber, M.* ; Gossler, A.* ; Lickert, H.

A novel Cre-inducible knock-in ARL13B-tRFP fusion cilium reporter.

Genesis 55:e23073 (2017)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Cilia play a major role in the regulation of numerous signaling pathways and are essential for embryonic development. Mutations in genes affecting ciliary function can cause a variety of diseases in humans summarized as ciliopathies. To facilitate the detection and visualization of cilia in a temporal and spatial manner in mouse tissues, we generated a Cre-inducible cilium-specific reporter mouse line expressing an ARL13B-tRFP fusion protein driven by a CMV enhancer/chicken β actin promotor (pCAG) from the Hprt locus. We detected bright and specific ciliary signals by immunostainings of various mono- and multiciliated tissues and by time-lapse live-cell analysis of cultured embryos and organ explant cultures. Additionally, we monitored cilium assembly and disassembly in embryonic fibroblast cells using live-cell imaging. Thus, the ARL13B-tRFP reporter mouse strain is a valuable tool for the investigation of ciliary structure and function in a tissue-specific manner to understand processes, such as ciliary protein trafficking or cilium-dependent signaling in vitro and in vivo.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Arl13b ; Cilia Inducible Transgene ; Ciliopathy ; Cilium Reporter ; Live Cell Imaging Of Cilia; Expression; Disease; Cells; Line
ISSN (print) / ISBN 1526-954X
e-ISSN 1526-968X
Quellenangaben Volume: 55, Issue: 11, Pages: , Article Number: e23073 Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Reviewing status Peer reviewed