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Mast cells acquire MHC II from dendritic cells during skin inflammation.

J. Exp. Med. 214, 3791-3811 (2017)
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Open Access Gold (Paid Option)
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as soon as is submitted to ZB.
Mast cells (MCs) and dendritic cells (DCs) are essential innate sentinels populating host-environment interfaces. Using longitudinal intravital multiphoton microscopy of DC GFP /MC RFP reporter mice, we herein provide in vivo evidence that migratory DCs execute targeted cell-to-cell interactions with stationary MCs before leaving the inflamed skin to draining lymph nodes. During initial stages of skin inflammation, DCs dynamically scan MCs, whereas at a later stage, long-lasting interactions predominate. These innate-to-innate synapse-like contacts ultimately culminate in DC-to-MC molecule transfers including major histocompatibility complex class II (MHCII) proteins enabling subsequent ex vivo priming of allogeneic T cells with a specific cytokine signature. The extent of MHCII transfer to MCs correlates with their T cell priming efficiency. Importantly, preventing the cross talk by preceding DC depletion decreases MC antigen presenting capacity and T cell-driven inflammation. Consequently, we identify an innate intercellular communication arming resident MCs with key DC functions that might contribute to the acute defense potential during critical periods of migration-based DC absence.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Allergic Contact-dermatitis; Cd8(+) T-cells; Class-ii; Elicitation Phase; In-vivo; Responses; Antibody; Hypersensitivity; Complexes; Immunity
Reviewing status
Institute(s) Institute for Pancreatic Beta Cell Research (IPI)