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Fallik, N.* ; Bar-Lavan, Y.* ; Greenshpan, Y.* ; Goldstein, O.* ; Grosch, M. ; Drukker, M. ; Gazit, R.*

Neat1 in hematopoietic stem cells.

Oncotarget 8, 109575-109586 (2017)
Verlagsversion Forschungsdaten DOI
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs (lncRNA) in HSCs. Neat1 is a lncRNA that plays a major role in the formation of sub-nuclear structures called paraspeckles, and was reported to regulate proliferation and differentiation in other cells types. We detected Neat1 expression using RNA-seq data and RT-qPCR in HSCs, progenitors and effector immune cells, by specific detection of its isoforms. Neat1 is highly expressed in stem and progenitor cells, yet it shows significant reduction in granulocytes. Microscopically, Neat1 is detected as sharp nuclear foci. Paraspeckle proteins NONO and PSPC1 are detected as aggregated nuclear foci in fresh primary hematopoietic cells, and in cultured cells. Induction of differentiation in vitro was found to enhance Neat1 expression. Taken together, our data demonstrate for the first time the expression of Neat1 and paraspeckles formation in HSCs and along hematopoiesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Neat1 ; Hematopoietic Stem Cells ; Paraspeckles ; Hematopoiesis ; Long Non-coding Rnas; Long Noncoding Rnas; Nuclear-bodies; Paraspeckles; Expression; Retention
ISSN (print) / ISBN 1949-2553
e-ISSN 1949-2553
Zeitschrift OncoTarget
Quellenangaben Band: 8, Heft: 65, Seiten: 109575-109586 Artikelnummer: , Supplement: ,
Verlag Impact Journals LLC
Verlagsort Orchard Park
Begutachtungsstatus Peer reviewed