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Analyses of HBV cccDNA quantification and modification.

Methods Mol. Biol. 1540, 59-72 (2017)
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Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Covalently Closed Circular Dna ; Deamination ; Differential Dna Denaturation Pcr ; Quantitative Pcr ; Quantitative Differential Dna Denaturation Pcr
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Bandtitel Hepatitis B Virus
Quellenangaben Band: 1540, Heft: , Seiten: 59-72 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Begutachtungsstatus Peer reviewed