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Hemmler, D.* ; Heinzmann, S.S. ; Wöhr, K.* ; Schmitt-Kopplin, P. ; Witting, M.

Tandem HILIC-RP liquid chromatography for increased polarity coverage in food analysis.

Electrophoresis 39, 1645-1653 (2018)
Verlagsversion Postprint DOI
Open Access Green
Comprehensive non-targeted analysis of food products normally requires two complementary chromatographic runs to achieve maximum compound coverage. In this study, we present a sensitive tandem-LC method, which combines RP and HILIC separation in a single run. The setup consists of a C18 trap column and two subsequently coupled analytical columns (HILIC and C18) which are operated in parallel. First, hydrophobic compounds are retained on the RP trap column while rather hydrophilic compounds are directly transferred onto a HILIC phase. Next, the pre-fractionated sample composition is analyzed by HILIC or RP chromatography, respectively. The presented setup allows individual and independent gradient elution as well as interfacing with mass spectrometry. The performance of the method has been proven by means of food relevant standards and analysis of complex food samples (e.g. red wine, meat extract). The simple and robust setup provides high flexibility in the selection of column combinations and does not require sophisticated instrumental setups or software. The method significantly increases the covered polarity range compared to classical one-dimensional chromatography. Our results indicate that tandem LC is a valuable and universal tool in the non-targeted screening of various types of complex food samples.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Foodomics ; Lc-ms ; Non-target Analysis ; Tandem Liquid Chromatography ; Wine; Polycyclic Aromatic-hydrocarbons; Lung Epithelial-cells; Yangtze-river Delta; 6 European Cities; Ambient Air; Oxidative Stress; Mouse Lung; A549 Cells; Cytotoxic Responses; Seasonal-variation
ISSN (print) / ISBN 0173-0835
e-ISSN 1522-2683
Zeitschrift Electrophoresis
Quellenangaben Band: 39, Heft: 13, Seiten: 1645-1653 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Po Box 211, 1000 Ae Amsterdam, Netherlands
Begutachtungsstatus Peer reviewed