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Micro-imaging of brain cancer radiotherapy using contrast computed tomography.
Int. J. Radiat. Oncol. Biol. Phys., accepted (2018)
DOI
online verfügbar vsl. 05/2019 
Purpose Experimental neuroimaging provides a wide range of methods for the visualization of brain anatomical morphology down to sub-cellular detail. Still, each technique-specific detection mechanism presents compromises between achievable field-of-view size, spatial resolution or nervous-tissue sensitivity, leading e.g. to partial sample coverage, unresolved morphological structure or sparse labeling of neuronal populations, and often also to obligatory sample dissections or other sample-invasive manipulations. X-ray Phase Contrast CT (PCI-CT) is an experimental imaging methodology, which simultaneously provides micrometric spatial resolution, high soft-tissue sensitivity and ex-vivo full-organ rodent brain coverage without any need for sample dissection, staining/labeling or contrast agent injection. In this work, we explore the benefits and limitations of PCI-CT use for the in-vitro imaging of normal and cancerous brain neuro-morphology after in-vivo treatment with synchrotron-generated X-ray Microbeam Radiation Therapy (MRT), a spatially fractionated experimental high-dose radiosurgery. The goals are the visualization of MRT treatment effects on nervous tissue, and qualitative comparison of results to histology and high-field MRI. Methods MRT was administered in-vivo to the brain of both healthy and cancer-bearing rats. Forty-five days post-treatment, brain organs were dissected out and imaged ex-vivo via propagation-based PCI-CT. Results PCI-CT visualizes brain anatomy and micro-vasculature in 3D, and distinguishes cancerous tissue morphology, necrosis, and intra-tumor accumulation of iron and calcium deposits. Moreover, PCI-CT detects the effects of MRT throughout treatment target areas, e.g. the formation of micrometer-thick radiation-induced tissue ablations. Observed neuro-structures were confirmed by histology and immunohistochemistry, and related to micro-MRI data. Conclusions PCI-CT enables a unique 3D neuroimaging approach for ex-vivo studies on small animal models, in that it concurrently delivers high-resolution insight on local brain tissue morphology in both normal and cancerous micro-milieu, localizes radiosurgical damage, and highlights deep micro-vasculature. This method could assist experimental small-animal neurology studies in the post-mortem evaluation of neuropathology or treatment effects.  
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 0360-3016
e-ISSN 0360-3016
Zeitschrift International Journal of Radiation Oncology, Biology, Physics
Verlag Elsevier
Begutachtungsstatus peer-reviewed