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Plass, M.* ; Solana, J.* ; Wolf, F.A. ; Ayoub, S.* ; Misios, A.* ; Glažar, P.* ; Obermayer, B.* ; Theis, F.J. ; Kocks, C.* ; Rajewsky, N.*

Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics.

Science 360:eaaq1723 (2018)
Publ. Version/Full Text Postprint DOI
Open Access Green
Flatworms of the species Schmidtea mediterranea are immortal-adult animals contain a large pool of pluripotent stem cells that continuously differentiate into all adult cell types. Therefore, single-cell transcriptome profiling of adult animals should reveal mature and progenitor cells. By combining perturbation experiments, gene expression analysis, a computational method that predicts future cell states from transcriptional changes, and a lineage reconstruction method, we placed all major cell types onto a single lineage tree that connects all cells to a single stem cell compartment. We characterized gene expression changes during differentiation and discovered cell types important for regeneration. Our results demonstrate the importance of single-cell transcriptome analysis for mapping and reconstructing fundamental processes of developmental and regenerative biology at high resolution.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Planarian Schmidtea-mediterranea; Stem-cells; Rna-seq; Gene-expression; Regeneration; Pluripotency; Trajectories; Organism; Tissues; System
ISSN (print) / ISBN 0036-8075
e-ISSN 1095-9203
Journal Science
Quellenangaben Volume: 360, Issue: 6391, Pages: , Article Number: eaaq1723 Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Publishing Place 1200 New York Ave, Nw, Washington, Dc 20005 Usa
Reviewing status