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Tsai, J.M.* ; Weissman, I.L.* ; Rinkevich, Y.

Partial lobular hepatectomy: A surgical model for morphologic liver regeneration.

J. Vis. Exp., DOI: 10.3791/57302 (2018)
Postprint DOI
Open Access Green
Morphological organ regeneration following acute tissue loss is common among lower vertebrates, but is rarely observed in mammalian postnatal life. Adult liver regeneration after 70% partial hepatectomy results in hepatocyte hypertrophy with some replication in remaining lobes with restoration of metabolic activity, but with permanent loss of the injured lobe's morphology and architecture. Here, we detail a new surgical method in the neonate that leaves a physiologic environment conducive to regeneration. This model involves amputation of the left lobe apex and a subsequent conservative management regimen, and lacks the necessity for ligation of major liver vessels or chemical injury, leaving a physiologic environment where regeneration may occur. We extend this protocol to amputations on juvenile (P7-14) mice, during which the injured liver transitions from organ regeneration to compensatory growth by hypertrophy. The presented, brief 30 min protocol provides a framework to study the mechanisms of regeneration, its age-associated decline in mammals, and the characterization of putative hepatic stem or progenitors.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Developmental Biology ; Issue 135 ; Partial Lobular Hepatectomy ; Hepatectomy ; Liver ; Liver Resection ; Liver Amputation ; Regeneration; Acetaminophen Hepatotoxicity; Mouse-liver; Mice; Toxicity; Hepatocytes; Injury; Cells
ISSN (print) / ISBN 1940-087X
e-ISSN 1940-087X
Quellenangaben Volume: , Issue: 135 Pages: , Article Number: , Supplement: ,
Publisher JoVE
Publishing Place 1 Alewife Center, Ste 200, Cambridge, Ma 02140 Usa
Reviewing status Peer reviewed