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Neuschmelting, V. ; Harmsen, S.* ; Bézière, N. ; Lockau, H.* ; Hsu, H.T.* ; Huang, R.C.* ; Razansky, D. ; Ntziachristos, V. ; Kircher, M.F.*

Dual-modality surface-enhanced resonance raman scattering and multispectral optoacoustic tomography nanoparticle approach for brain tumor delineation.

Small 14:e1800740 (2018)
Open Access Green as soon as Postprint is submitted to ZB.
Ambient inhalable particulate matter (PM) is a serious health concern worldwide, but especially so in China where high PM concentrations affect huge populations. Atmospheric processes and emission sources cause spatial and temporal variations in PM concentration and chemical composition, but their influence on the toxicological characteristics of PM are still inadequately understood.In this study, we report an extensive chemical and toxicological characterization of size-segregated urban air inhalable PM collected in August and October 2013 from Nanjing, and assess the effects of atmospheric processes and likely emission sources. A549 human alveolar epithelial cells were exposed to day- and nighttime PM samples (25, 75, 150, 200, 300 mu g/ml) followed by analyses of cytotoxicity, genotoxicity, cell cycle, and inflammatory response.PM10-2.5 and PM0.2 caused the greatest toxicological responses for different endpoints, illustrating that particles with differing size and chemical composition activate distinct toxicological pathways in A549 cells. PM10-2.5 displayed the greatest oxidative stress and genotoxic responses; both were higher for the August samples compared with October. In contrast, PM0.2 and PM2.5-1.0 samples displayed high cytotoxicity and substantially disrupted cell cycle; August samples were more cytotoxic whereas October samples displayed higher cell cycle disruption. Several components associated with combustion, traffic, and industrial emissions displayed strong correlations with these toxicological responses. The lower responses for PM1.0-0.2 compared to PM0.2 and PM2.5-1.0 indicate diminished toxicological effects likely due to aerosol aging and lower proportion of fresh emission particles rich in highly reactive chemical components in the PM1.0-0.2 fraction.Different emission sources and atmospheric processes caused variations in the chemical composition and toxicological responses between PM fractions, sampling campaigns, and day and night. The results indicate different toxicological pathways for coarse-mode particles compared to the smaller particle fractions with typically higher content of combustion-derived components. The variable responses inside PM fractions demonstrate that differences in chemical composition influence the induced toxicological responses.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Glioblastoma ; Multispectral Optoacoustic Tomography (msot) ; Nanomedicine ; Neurooncology ; Photoacoustic Imaging ; Surface-enhanced Resonance Raman Spectroscopy (serrs); Polycyclic Aromatic-hydrocarbons; Lung Epithelial-cells; Yangtze-river Delta; 6 European Cities; Ambient Air; Oxidative Stress; Mouse Lung; A549 Cells; Cytotoxic Responses; Seasonal-variation
ISSN (print) / ISBN 1613-6810
e-ISSN 1613-6829
Journal Small
Quellenangaben Volume: 14, Issue: 23, Pages: , Article Number: e1800740 Supplement: ,
Publisher Wiley
Publishing Place Po Box 211, 1000 Ae Amsterdam, Netherlands
Reviewing status Peer reviewed