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Makowski, M.M.* ; Grawe, C.* ; Foster, B. ; Nguyen, N.V.* ; Bartke, T. ; Vermeulen, M.*

Global profiling of protein-DNA and protein-nucleosome binding affinities using quantitative mass spectrometry.

Nat. Commun. 9:1653 (2018)
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Open Access Gold
Creative Commons Lizenzvertrag
Interaction proteomics studies have provided fundamental insights into multimeric biomolecular assemblies and cell-scale molecular networks. Significant recent developments in mass spectrometry-based interaction proteomics have been fueled by rapid advances in label-free, isotopic, and isobaric quantitation workflows. Here, we report a quantitative protein-DNA and protein-nucleosome binding assay that uses affinity purifications from nuclear extracts coupled with isobaric chemical labeling and mass spectrometry to quantify apparent binding affinities proteome-wide. We use this assay with a variety of DNA and nucleosome baits to quantify apparent binding affinities of monomeric and multimeric transcription factors and chromatin remodeling complexes.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Randomized Controlled-trial; Chronic Tic Disorders; Parkinsons-disease; Health-care; Tourette-syndrome; Behavior-therapy; Neurological Disorders; Huntington Disease; Batten-disease; Virtual Visits
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 9, Issue: 1, Pages: , Article Number: 1653 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed