PuSH - Publication Server of Helmholtz Zentrum München

Völker-Albert, M.C.* ; Schmidt, A.* ; Barth, T.K. ; Forne, I.* ; Imhof, A.*

Detection of histone modification dynamics during the cell cycle by MS-based proteomics.

Methods Mol. Biol. 1832, 61-74 (2018)
Open Access Green as soon as Postprint is submitted to ZB.
DNA replication and subsequent deposition of nucleosomes is critical for the maintenance of the genome and epigenetic inheritance. Experiments using human tissue culture cells harvested at defined stages of the cell cycle can help to elucidate the mechanism of histone deposition and chromatin assembly in detail. Here, we describe a pulsed-SILAC approach to distinguish newly synthesized and deposited histones during S-phase of the cell cycle from parental “old” histones incorporated in previous replications and to decipher posttranslational histone modifications (PTMs).
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords Histone Quantitation ; Mass Spectrometry ; Ptm-analysis ; Silac Labeling
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Quellenangaben Volume: 1832, Issue: , Pages: 61-74 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed