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Detection of histone modification dynamics during the cell cycle by MS-based proteomics.
Methods Mol. Biol. 1832, 61-74 (2018)
DNA replication and subsequent deposition of nucleosomes is critical for the maintenance of the genome and epigenetic inheritance. Experiments using human tissue culture cells harvested at defined stages of the cell cycle can help to elucidate the mechanism of histone deposition and chromatin assembly in detail. Here, we describe a pulsed-SILAC approach to distinguish newly synthesized and deposited histones during S-phase of the cell cycle from parental “old” histones incorporated in previous replications and to decipher posttranslational histone modifications (PTMs).
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Publication type Article: Journal article
Document type Scientific Article
Keywords Histone Quantitation ; Mass Spectrometry ; Ptm-analysis ; Silac Labeling
Institute(s) Research Unit Protein Science (PROT)