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Essig, K.* ; Kronbeck, N.* ; Guimaraes, J.C.* ; Lohs, C. ; Schlundt, A. ; Hoffmann, A.L.* ; Behrens, G.* ; Brenner, S. ; Kowalska, J.* ; Lopez-Rodriguez, C.* ; Jemielity, J.* ; Holtmann, H.* ; Reiche, K.* ; Hackermüller, J.* ; Sattler, M. ; Zavolan, M.* ; Heissmeyer, V.

Roquin targets mRNAs in a 3 '-UTR-specific manner by different modes of regulation.

Nat. Commun. 9:3810 (2018)
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The RNA-binding proteins Roquin-1 and Roquin-2 redundantly control gene expression and cell-fate decisions. Here, we show that Roquin not only interacts with stem-loop structures, but also with a linear sequence element present in about half of its targets. Comprehensive analysis of a minimal response element of the Nfkbid 3'-UTR shows that six stem-loop structures cooperate to exert robust and profound post-transcriptional regulation. Only binding of multiple Roquin proteins to several stem-loops exerts full repression, which redundantly involved deadenylation and decapping, but also translational inhibition. Globally, most Roquin targets are regulated by mRNA decay, whereas a small subset, including the Nfat5 mRNA, with more binding sites in their 3'-UTRs, are also subject to translational inhibition. These findings provide insights into how the robustness and magnitude of Roqu-inmediated regulation is encoded in complex cis-elements.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Posttranscriptional Gene-regulation; Constitutive-decay Element; Pathogenic T(h)17 Cells; Transcription Factor; Noncoding Rna; T-cells; In-vivo; Th17; Inflammation; Autoimmunity
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 9, Issue: 1, Pages: , Article Number: 3810 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed