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Higareda-Almaraz, J. ; Karbiener, M.* ; Giroud, M. ; Pauler, F.M.* ; Gerhalter, T.* ; Herzig, S. ; Scheideler, M.

Norepinephrine triggers an immediate-early regulatory network response in primary human white adipocytes.

BMC Genomics 19:794 (2018)
Verlagsversion DOI
Open Access Gold
Creative Commons Lizenzvertrag
Background: Norepinephrine (NE) signaling has a key role in white adipose tissue (WAT) functions, including lipolysis, free fatty acid liberation and, under certain conditions, conversion of white into brite (brown-in-white) adipocytes. However, acute effects of NE stimulation have not been described at the transcriptional network level.Results: We used RNA-seq to uncover a broad transcriptional response. The inference of protein-protein and protein-DNA interaction networks allowed us to identify a set of immediate-early genes (IEGs) with high betweenness, validating our approach and suggesting a hierarchical control of transcriptional regulation. In addition, we identified a transcriptional regulatory network with IEGs as master regulators, including HSF1 and NFIL3 as novel NE-induced IEG candidates. Moreover, a functional enrichment analysis and gene clustering into functional modules suggest a crosstalk between metabolic, signaling, and immune responses.Conclusions: Altogether, our network biology approach explores for the first time the immediate-early systems level response of human adipocytes to acute sympathetic activation, thereby providing a first network basis of early cell fate programs and crosstalks between metabolic and transcriptional networks required for proper WAT function.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Norepinephrine Stimulation ; White Adipocyte ; Early Cell Fate ; Network Biology ; Immediate-early Gene ; Transcriptional Regulatory Network; Brown Adipose-tissue; Mesenchymal Stem-cells; Adipogenic Differentiation; Induced Thermogenesis; Gene-expression; Activation; Pathways; Fat; Growth; Distinct
ISSN (print) / ISBN 1471-2164
e-ISSN 1471-2164
Zeitschrift BMC Genomics
Quellenangaben Band: 19, Heft: 1, Seiten: , Artikelnummer: 794 Supplement: ,
Verlag BioMed Central
Verlagsort Campus, 4 Crinan St, London N1 9xw, England
Begutachtungsstatus Peer reviewed