PuSH - Publikationsserver des Helmholtz Zentrums München

Whole-cell photoacoustic sensor based on pigment relocalization.

ACS sens. 4, 603-612 (2019)
Verlagsversion Forschungsdaten DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Photoacoustic (optoacoustic) imaging can extract molecular information with deeper tissue penetration than possible by fluorescence microscopy techniques. However, there is currently still a lack of robust genetically controlled contrast agents and molecular sensors that can dynamically detect biological analytes of interest with photoacoustics. In a biomimetic approach, we took inspiration from cuttlefish who can change their color by relocalizing pigment-filled organelles in so-called chromatophore cells under neurohumoral control. Analogously, we tested the use of melanophore cells from Xenopus laevis, containing compartments (melanosomes) filled with strongly absorbing melanin, as whole-cell sensors for optoacoustic imaging. Our results show that pigment relocalization in these cells, which is dependent on binding of a ligand of interest to a specific G protein-coupled receptor (GPCR), can be monitored in vitro and in vivo using photoacoustic mesoscopy. In addition to changes in the photoacoustic signal amplitudes, we could furthermore detect the melanosome aggregation process by a change in the frequency content of the photoacoustic signals. Using bioinspired engineering, we thus introduce a photoacoustic pigment relocalization sensor (PaPiReS) for molecular photoacoustic imaging of GPCR-mediated signaling molecules.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Photoacoustics ; Optoacoustics ; Whole-cell Sensor ; G Protein-coupled Receptor Signaling ; Gpcr Signaling ; Melanophores ; Photoacoustic Frequency Spectrum ; Molecular Imaging; Dispersion; Melanophores; Aggregation; Tomography; Expression; Dopamine; Receptor; Light
ISSN (print) / ISBN 2379-3694
e-ISSN 2379-3694
Zeitschrift ACS sensors
Quellenangaben Band: 4, Heft: 3, Seiten: 603-612 Artikelnummer: , Supplement: ,
Verlag ACS
Verlagsort Washington, DC
Begutachtungsstatus Peer reviewed