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Heimbeck, I. ; Hofer, T.P. ; Eder, C. ; Wright, A.K.* ; Frankenberger, M. ; Marei, A. ; Boghdadi, G.* ; Scherberich, J.* ; Ziegler-Heitbrock, L.

Standardized single-platform assay for human monocyte subpopulations: Lower CD14⁺CD16⁺⁺ monocytes in females.

Cytometry A 77A, 823-830 (2010)
Verlagsversion DOI
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
We present a novel single-platform assay for determination of the absolute number of human blood monocyte subpopulations, i.e., the CD14(++)CD16(-) and the CD14(+)CD16(++) monocytes. A four-color combination of antibodies to CD14, CD16, CD45, and HLA-DR reduces the spill-over of natural killer cells and of granulocytes into the CD14(+)CD16(++) monocyte gate. For these CD14(+)CD16(++) monocytes, the intra-assay coefficient of variation (CV) was 4.1% and the inter-assay CV was 8.5%. Looking at a cohort of 40 donors aged 18-60 years, we found no age dependence. There was however an effect of gender in that females had lower CD14(+)CD16(++) monocytes (45.4 +/- 13.5 cells/microl) compared with males (59.1 +/- 20.3 cells/microl) (P < 0.02). Using this novel approach, we can confirm that exercise will lead to more than three-fold increase of the CD14(+)CD16(++) monocytes. Also, we show that therapy with low doses of glucocorticoids will deplete these cells. This robust single-platform assay may be a useful tool for monitoring the absolute number of monocyte subpopulations in health and disease.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter monocyte heterogeneity; gender; exercise; glucocorticoids
ISSN (print) / ISBN 1552-4922
e-ISSN 1552-4930
Zeitschrift Cytometry Part A
Quellenangaben Band: 77A, Heft: 9, Seiten: 823-830 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken, NJ
Institut(e) CCG Inflammatory Lung Diseases (CPC-KEL)