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Duin, S.* ; Schütz, K.* ; Ahlfeld, T.* ; Lehmann, S. ; Lode, A.* ; Ludwig, B. ; Gelinsky, M.*

3D bioprinting of functional islets of Langerhans in an alginate/methylcellulose hydrogel blend.

Adv. Healthc. Mater. 8:e1801631 (2019)
Publ. Version/Full Text DOI
Open Access Green as soon as Postprint is submitted to ZB.
Transplantation of pancreatic islets is a promising strategy to alleviate the unstable blood-glucose control that some patients with diabetes type 1 exhibit and has seen many advances over the years. Protection of transplanted islets from the immune system can be accomplished by encapsulation within a hydrogel, the most investigated of which is alginate. In this study, islet encapsulation is combined with 3D extrusion bioprinting, an additive manufacturing method which enables the fabrication of 3D structures with a precise geometry to produce macroporous hydrogel constructs with embedded islets. Using a plottable hydrogel blend consisting of clinically approved ultrapure alginate and methylcellulose (Alg/MC) enables encapsulating pancreatic islets in macroporous 3D hydrogel constructs of defined geometry while retaining their viability, morphology, and functionality. Diffusion of glucose and insulin in the Alg/MC hydrogel is comparable to diffusion in plain alginate; the embedded islets continuously produce insulin and glucagon throughout the observation and still react to glucose stimulation albeit to a lesser degree than control islets.
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Publication type Article: Journal article
Document type Scientific Article
Keywords 3d Bioprinting ; Alginate ; Diabetes ; Insulin ; Macroporous ; Pancreatic Islet; Pancreatic-islets; In-vitro; Rat Islets; Nonimmunosuppressed Patients; Insulin-secretion; Beta-cells; Adult-rat; Alginate; Transplantation; Encapsulation
ISSN (print) / ISBN 2192-2640
e-ISSN 2192-2659
Quellenangaben Volume: 8, Issue: 7, Pages: , Article Number: e1801631 Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Reviewing status Peer reviewed
Institute(s) Institute for Pancreatic Beta Cell Research (IPI)