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Peters, L.* ; Weidenfeld, I. ; Klemm, U. ; Loeschcke, A.* ; Weihmann, R.* ; Jaeger, K.E.* ; Drepper, T.* ; Ntziachristos, V. ; Stiel, A.-C.

Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity.

Nat. Commun. 10:1191 (2019)
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Open Access Gold
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The morphology, physiology and immunology, of solid tumors exhibit spatial heterogeneity which complicates our understanding of cancer progression and therapy response. Understanding spatial heterogeneity necessitates high resolution in vivo imaging of anatomical and pathophysiological tumor information. We introduce Rhodobacter as bacterial reporter for multispectral optoacoustic (photoacoustic) tomography (MSOT). We show that endogenous bacteriochlorophyll a in Rhodobacter gives rise to strong optoacoustic signals >800 nm away from interfering endogenous absorbers. Importantly, our results suggest that changes in the spectral signature of Rhodobacter which depend on macrophage activity inside the tumor can be used to reveal heterogeneity of the tumor microenvironment. Employing non-invasive high resolution MSOT in longitudinal studies we show spatiotemporal changes of Rhodobacter spectral profiles in mice bearing 4T1 and CT26.WT tumor models. Accessibility of Rhodobacter to genetic modification and thus to sensory and therapeutic functions suggests potential for a theranostic platform organism.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Tumor Microenvironment; Rhodobacter-sphaeroides; Rhodospirillum-centenum; Expression; Bacteriochlorophyll; Cancer; Capsulatus; Therapy; Genes; Lipopolysaccharide
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 10, Issue: 1, Pages: , Article Number: 1191 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed