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Structural insights into BET client recognition of endometrial and prostate cancer-associated SPOP mutants.

J. Mol. Biol. 431, 2213-2221 (2019)
Postprint Research data DOI
Open Access Green
BET proteins such as BRD3 are oncogenic transcriptional coactivators. SPOP binding triggers their proteasomal degradation. In both endometrial and prostate cancers, SPOP mutations occur in the MATH domain, but with opposed influence on drug susceptibility. In prostate cancer, SPOP mutations presumably cause increased BET levels, decreasing BET inhibitor drug susceptibility. As opposed, in endometrial cancer, decreased BET levels concomitant with higher BET inhibitor drug susceptibility were observed. Here, we present the to our knowledge first co-crystal structure of SPOP and a bromodomain containing protein (BRD3). Our structural and biophysical data confirm the suggested loss-of-function in prostate cancer-associated SPOP mutants and provide mechanistic explanation. As opposed to previous literature, our data on endometrial cancer-associated SPOP mutants do not show altered binding behavior compared to wild-type SPOP, indicating a more complex regulatory mechanism. SPOP mutation screening may thus be considered a valuable personalized medicine tool for effective antitumor therapy. (C) 2019 Elsevier Ltd. All rights reserved.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Spop ; Bet Protein ; Cancer ; Ubiquitination ; X-ray Crystallograpgy; Mutations; Stabilization; Resistance; Inhibitors
ISSN (print) / ISBN 0022-2836
e-ISSN 1089-8638
Quellenangaben Volume: 431, Issue: 11, Pages: 2213-2221 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place 24-28 Oval Rd, London Nw1 7dx, England
Reviewing status Peer reviewed
Institute(s) Institute of Structural Biology (STB)
Institute of Medicinal Chemistry (IMC)