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Schneider, A.* ; Kurz, S.* ; Manske, K.* ; Janas, M.K.* ; Heikenwälder, M.* ; Misgeld, T.* ; Aichler, M. ; Weissmann, S.F.* ; Zischka, H. ; Knolle, P.* ; Wohlleber, D.*

Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.

Sci. Rep. 9:8492 (2019)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
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Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Flow-cytometry; Cultured-cells; Protein; Mechanisms; Mitophagy; Dynamics; Immunity; Disease; Model; Mavs
Reviewing status