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Single organelle analysis to characterize mitochondrial function and crosstalk during viral infection.

Sci. Rep. 9:8492 (2019)
Publishers Version Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
as soon as is submitted to ZB.
Mitochondria are key for cellular metabolism and signalling processes during viral infection. We report a methodology to analyse mitochondrial properties at the single-organelle level during viral infection using a recombinant adenovirus coding for a mitochondrial tracer protein for tagging and detection by multispectral flow cytometry. Resolution at the level of tagged individual mitochondria revealed changes in mitochondrial size, membrane potential and displayed a fragile phenotype during viral infection of cells. Thus, single-organelle and multi-parameter resolution allows to explore altered energy metabolism and antiviral defence by tagged mitochondria selectively in virus-infected cells and will be instrumental to identify viral immune escape and to develop and monitor novel mitochondrial-targeted therapies.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Flow-cytometry; Cultured-cells; Protein; Mechanisms; Mitophagy; Dynamics; Immunity; Disease; Model; Mavs
Reviewing status