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Vieira Braga, F.A.* ; Kar, G.* ; Berg, M.* ; Carpaij, O.A.* ; Polanski, K.* ; Simon, L. ; Brouwer, S.* ; Gomes, T.* ; Hesse, L.* ; Jiang, J.* ; Fasouli, E.S.* ; Efremova, M.* ; Vento-Tormo, R.* ; Talavera-López, C.* ; Jonker, M.R.* ; Affleck, K.* ; Palit, S. ; Strzelecka, P.M.* ; Firth, H.V.* ; Mahbubani, K.T.* ; Cvejic, A.* ; Meyer, K.B.* ; Saeb-Parsy, K.* ; Luinge, M.* ; Brandsma, C.A.* ; Timens, W.* ; Angelidis, I. ; Strunz, M. ; Koppelman, G.H.* ; van Oosterhout, A.J.* ; Schiller, H. B. ; Theis, F.J. ; van den Berge, M.* ; Nawijn, M.C.* ; Teichmann, S.A.*

A cellular census of human lungs identifies novel cell states in health and in asthma.

Nat. Med. 25, 1153-1163 (2019)
Publ. Version/Full Text Postprint Research data DOI
Open Access Green
Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (T(H)2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a T(H)2-dominated interactome in asthmatic lungs.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Epithelial-cells; Prostaglandin D-2; Rna-seq; Expression; Inflammation; Pathogenesis; Macrophages; Phenotype; Disease; Gene
ISSN (print) / ISBN 1078-8956
e-ISSN 1546-170X
Journal Nature medicine
Quellenangaben Volume: 25, Issue: 7, Pages: 1153-1163 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed