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Kalde, M.* ; Elliott, L.* ; Ravikumar, R.* ; Rybak, K.* ; Altmann, M. ; Klaeger, S.* ; Wiese, C.* ; Abele, M.* ; Al, B.* ; Kalbfuß, N.* ; Qi, X.* ; Steiner, A.* ; Meng, C.* ; Zheng, H.* ; Kuster, B.* ; Falter-Braun, P. ; Ludwig, C.* ; Moore, I.* ; Assaad, F.F.*

Interactions between Transport Protein Particle (TRAPP) complexes and Rab GTPases in Arabidopsis.

Plant J. 100, 279-297 (2019)
Postprint DOI
Open Access Green
Transport Protein Particle II (TRAPPII) is essential for exocytosis, endocytosis, protein sorting and cytokinesis. In spite of a considerable understanding of its biological role, little information is known about Arabidopsis TRAPPII complex topology and molecular function. In this study, independent proteomic approaches initiated with TRAPP components or Rab-A GTPase variants converge on the TRAPPII complex. We show that the Arabidopsis genome encodes the full complement of 13 TRAPPC subunits, including four previously unidentified components. A dimerization model is proposed to account for binary interactions between TRAPPII subunits. Preferential binding to dominant negative (GDP-bound) versus wild-type or constitutively active (GTP-bound) RAB-A2a variants discriminates between TRAPPII and TRAPPIII subunits and shows that Arabidopsis complexes differ from yeast but resemble metazoan TRAPP complexes. Analyzes of Rab-A mutant variants in trappii backgrounds provide genetic evidence that TRAPPII functions upstream of RAB-A2a, allowing us to propose that TRAPPII is likely to behave as a guanine nucleotide exchange factor (GEF) for the RAB-A2a GTPase. GEFs catalyze exchange of GDP for GTP; the GTP-bound, activated, Rab then recruits a diverse local network of Rab effectors to specify membrane identity in subsequent vesicle fusion events. Understanding GEF-Rab interactions will be crucial to unravel the co-ordination of plant membrane traffic.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Arabidopsis Thaliana ; Cytokinesis ; Rab Gtpases ; Secretory Pathway ; Tethering Complexes; Nucleotide Exchange Factor; Bovine Brain Cytosol; Golgi Network; Regulates Autophagy; Subsequent Binding; Sequential Action; Plasma-membrane; Cytokinesis; Trafficking; Gene
ISSN (print) / ISBN 0960-7412
e-ISSN 1365-313X
Quellenangaben Volume: 100, Issue: 2, Pages: 279-297 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Reviewing status Peer reviewed
Institute(s) Institute of Network Biology (INET)