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Xue, K. ; Mamone, S.* ; Koch, B. ; Sarkar, R. ; Reif, B.

Determination of methyl order parameters using solid state NMR under off magic angle spinning.

J. Biomol. NMR 73, 471-475 (2019)
Postprint DOI Order publishers version
Open Access Green
Quantification of dipolar couplings in biological solids is important for the understanding of dynamic processes. Under Magic Angle Spinning (MAS), order parameters are normally obtained by recoupling of anisotropic interactions involving the application of radio frequency pulses. We have recently shown that amide backbone order parameters can be estimated accurately in a spin-echo experiment in case the rotor spinning angle is slightly mis-calibrated. In this work, we apply this method to determine methyl order parameters in a deuterated sample of the SH3 domain of chicken a-spectrin in which the methyl containing side chains valine and leucine are selectively protonated.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Solid-state Nmr ; Off Magic Angle Spinning ; Spin Echo ; Microcrystalline Proteins ; Perdeuteration; Nuclear-magnetic-resonance; Side-chain Dynamics; Model-free Approach; Backbone Dynamics; Dipolar Couplings; Staphylococcal Nuclease; Relaxation; Protein; Spectroscopy; Spectra
ISSN (print) / ISBN 0925-2738
e-ISSN 1573-5001
Quellenangaben Volume: 73, Issue: 8-9, Pages: 471-475 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands
Reviewing status Peer reviewed