PuSH - Publikationsserver des Helmholtz Zentrums München

Friberg, A. ; Oddone, A.* ; Klymenko, T.* ; Müller, J.* ; Sattler, M.

Structure of an atypical Tudor domain in the Drosophila Polycomblike protein.

Protein Sci. 19, 1906-1916 (2010)
Verlagsversion DOI
Free by publisher
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Post-translational modifications of histone tails are among the most prominent epigenetic marks and play a critical role in transcriptional control at the level of chromatin. The Polycomblike (Pcl) protein is part of a histone methyltransferase complex (Pcl-PRC2) responsible for high levels of histone H3 K27 trimethylation. Studies in Drosophila larvae suggest that Pcl is required for anchoring Pcl-PRC2 at target genes, but how this is achieved is unknown. Pcl comprises a Tudor domain and two PHD fingers. These domains are known to recognize methylated lysine or arginine residues and could contribute to targeting of Pcl-PRC2. Here, we report an NMR structure of the Tudor domain from Drosophila Pcl (Pcl-Tudor) and binding studies with putative ligands. Pcl-Tudor contains an atypical, incomplete aromatic cage that does not interact with known Tudor domain ligands, such as methylated lysines or arginines. Interestingly, human Pcl orthologs exhibit a complete aromatic cage, suggesting that they may recognize methylated lysines. Structural comparison with other Tudor domains suggests that Pcl-Tudor may engage in intra- or intermolecular interactions through an exposed hydrophobic surface patch.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter NMR; Polycomblike; Pcl; PRC2; Tudor; Aromatic cage; Methyllysine; sDMA; Post-translational modification; Transcriptional regulation
ISSN (print) / ISBN 0961-8368
e-ISSN 1469-896X
Zeitschrift Protein Science
Quellenangaben Band: 19, Heft: 10, Seiten: 1906-1916 Artikelnummer: , Supplement: ,
Verlag Wiley
Begutachtungsstatus Peer reviewed