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Gutmann, T. ; Schäfer, I.B.* ; Poojari, C.* ; Brankatschk, B. ; Vattulainen, I.* ; Strauss, M.* ; Coskun, Ü.

Cryo-EM structure of the complete and ligand-saturated insulin receptor ectodomain.

J. Cell Biol. 219:e201907210 (2020)
Verlagsversion DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Glucose homeostasis and growth essentially depend on the hormone insulin engaging its receptor. Despite biochemical and structural advances, a fundamental contradiction has persisted in the current understanding of insulin ligand-receptor interactions. While biochemistry predicts two distinct insulin binding sites, 1 and 2, recent structural analyses have resolved only site 1. Using a combined approach of cryo-EM and atomistic molecular dynamics simulation, we present the structure of the entire dimeric insulin receptor ectodomain saturated with four insulin molecules. Complementing the previously described insulin-site 1 interaction, we present the first view of insulin bound to the discrete insulin receptor site 2. Insulin binding stabilizes the receptor ectodomain in a T-shaped conformation wherein the membrane-proximal domains converge and contact each other. These findings expand the current models of insulin binding to its receptor and of its regulation. In summary, we provide the structural basis for a comprehensive description of ligand-receptor interactions that ultimately will inform new approaches to structure-based drug design.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Real-space Refinement; Particle Mesh Ewald; Negative Cooperativity; Quaternary Structure; Linked Glycans; Egf Receptor; Binding; Protein; Dynamics; Domain
ISSN (print) / ISBN 0021-9525
e-ISSN 1540-8140
Quellenangaben Band: 219, Heft: 1, Seiten: , Artikelnummer: e201907210 Supplement: ,
Verlag Rockefeller University Press
Verlagsort 950 Third Ave, 2nd Flr, New York, Ny 10022 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Pancreatic Beta Cell Research (IPI)