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Dugué, P.A.* ; Wilson, R. ; Lehne, B.* ; Jayasekara, H.* ; Wang, X.* ; Jung, C.H.* ; Joo, J.E.* ; Makalic, E.* ; Schmidt, D.F.* ; Baglietto, L.* ; Severi, G.* ; Gieger, C. ; Ladwig, K.-H. ; Peters, A. ; Kooner, J.S.* ; Southey, M.C.* ; English, D.R.* ; Waldenberger, M. ; Giles, G.G.* ; Milne, R.L.*

Alcohol consumption is associated with widespread changes in blood DNA methylation: Analysis of cross-sectional and longitudinal data.

Addict. Biol. 26:e12855 (2021)
Verlagsversion Postprint Forschungsdaten DOI
Open Access Green
DNA methylation may be one of the mechanisms by which alcohol consumption is associated with the risk of disease. We conducted a large-scale, cross-sectional, genome-wide DNA methylation association study of alcohol consumption and a longitudinal analysis of repeated measurements taken several years apart. Using the Illumina HumanMethylation450 BeadChip, DNA methylation was measured in blood samples from 5606 Melbourne Collaborative Cohort Study (MCCS) participants. For 1088 of them, these measures were repeated using blood samples collected a median of 11 years later. Associations between alcohol intake and blood DNA methylation were assessed using linear mixed-effects regression models. Independent data from the London Life Sciences Prospective Population (LOLIPOP) (N = 4042) and Cooperative Health Research in the Augsburg Region (KORA) (N = 1662) cohorts were used to replicate associations discovered in the MCCS. Cross-sectional analyses identified 1414 CpGs associated with alcohol intake at P < 10−7, 1243 of which had not been reported previously. Of these novel associations, 1078 were replicated (P <.05) using LOLIPOP and KORA data. Using the MCCS data, we also replicated 403 of 518 previously reported associations. Interaction analyses suggested that associations were stronger for women, non-smokers, and participants genetically predisposed to consume less alcohol. Of the 1414 CpGs, 530 were differentially methylated (P <.05) in former compared with current drinkers. Longitudinal associations between the change in alcohol intake and the change in methylation were observed for 513 of the 1414 cross-sectional associations. Our study indicates that alcohol intake is associated with widespread changes in DNA methylation across the genome. Longitudinal analyses showed that the methylation status of alcohol-associated CpGs may change with alcohol consumption changes in adulthood.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Alcohol Consumption ; Cross-sectional Data ; Dna Methylation ; Epigenome-wide Association Study ; Ewas ; Hm450 Assay ; Longitudinal Data; Epigenome-wide Association; Peripheral-blood; Cancer Risk; Epigenetics; Metabolism; Mechanisms; Dependence; Promoter
ISSN (print) / ISBN 1355-6215
e-ISSN 1369-1600
Zeitschrift Addiction Biology
Quellenangaben Band: 26, Heft: , Seiten: , Artikelnummer: e12855 Supplement: ,
Verlag Wiley
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Begutachtungsstatus Peer reviewed