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Glucose homeostasis is regulated by pancreatic beta-cell cilia via endosomal EphA-processing.

Nat. Commun. 10:5686 (2019)
Publ. Version/Full Text Research data DOI
Open Access Gold
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Diabetes mellitus affects one in eleven adults worldwide. Most suffer from Type 2 Diabetes which features elevated blood glucose levels and an inability to adequately secrete or respond to insulin. Insulin producing beta-cells have primary cilia which are implicated in the regulation of glucose metabolism, insulin signaling and secretion. To better understand how beta-cell cilia affect glucose handling, we ablate cilia from mature beta-cells by deleting key cilia component Ift88. Here we report that glucose homeostasis and insulin secretion deteriorate over 12 weeks post-induction. Cilia/basal body components are required to suppress spontaneous auto-activation of EphA3 and hyper-phosphorylation of EphA receptors inhibits insulin secretion. In beta-cells, loss of cilia/basal body function leads to polarity defects and epithelial-to-mesenchymal transition. Defective insulin secretion from IFT88-depleted human islets and elevated pEPHA3 in islets from diabetic donors both point to a role for cilia/basal body proteins in human glucose homeostasis.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Bardet-biedl-syndrome; Insulin-secretion; Disruption; Receptor; Gtpase; Islets; Mouse; Mass; Endocytosis; Proteins
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 10, Issue: 1, Pages: , Article Number: 5686 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed