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Qian, Z.* ; Bruhn, T.* ; D'Agostino, P.M.* ; Herrmann, A. ; Haslbeck, M.* ; Antal, N.* ; Fiedler, H.P.* ; Brack-Werner, R. ; Gulder, T.A.M.*

Discovery of the streptoketides by direct cloning and rapid heterologous expression of a cryptic PKS II gene cluster from streptomyces sp. Tü 6314.

J. Org. Chem. 85, 664-673 (2020)
Verlagsversion Postprint DOI
Open Access Green
Genome sequencing and bioinformatic analysis have identified numerous cryptic gene clusters that have the potential to produce novel natural products. Within this work, we identified a cryptic type II PKS gene cluster (skt) from Streptomyces sp. Tu 6314. Facilitated by linear plus linear homologous recombination-mediated recombineering (LLHR), we directly cloned the skt gene cluster using the Streptomyces site-specific integration vector pSET152. Direct cloning allowed for rapid heterologous expression in Streptomyces coelicolor, leading to the identification and structural characterization of six polyketides (three known compounds and new streptoketides), four of which exhibit anti-HIV activities. Our study shows that the pSET152 vector can be directly used for LLHR, expanding the Rec/ET direct cloning toolbox and providing the possibility for rapid heterologous expression of gene clusters from Streptomyces.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Microbial Natural-products; Starter Unit; Polyketide Synthase; Biosynthesis; Daunorubicin; Dna; Anthracycline; Ketosynthase; Doxorubicin; Coelicolor
ISSN (print) / ISBN 0022-3263
e-ISSN 1520-6904
Quellenangaben Band: 85, Heft: 2, Seiten: 664-673 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort 1155 16th St, Nw, Washington, Dc 20036 Usa
Begutachtungsstatus Peer reviewed