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Cota, P. ; Helmi, S.A.* ; Hsu, C.* ; Rancourt, D.E.*

Cytokine directed chondroblast trans-differentiation: JAK inhibition facilitates direct reprogramming of fibroblasts to chondroblasts.

Cells 9:191 (2020)
Verlagsversion DOI
Open Access Gold
Creative Commons Lizenzvertrag
Osteoarthritis (OA) is a degenerative disease of the hyaline articular cartilage. This disease is progressive and may lead to disability. Researchers proposed many regenerative approaches to treat osteoarthritis, including stem cells. Trans-differentiation of a fully differentiated cell state directly into another different differentiated cell state avoids the disadvantages of fully reprogramming cells to induced pluripotent stem cells (iPSCs) in terms of faster reprogramming of the needed cells. Trans-differentiation also reduces the risk of tumor formation by avoiding the iPSC state. OSKM factors (Oct4, Sox2, Klf4, and cMyc) accompanied by the JAK-STAT pathway inhibition, followed by the introduction of specific differentiation factors, directly reprogrammed mouse embryonic fibroblasts to chondroblasts. Our results showed the absence of intermediate induced pluripotent stem cell formation. The resulting aggregates showed clear hyaline and hypertrophic cartilage. Tumor formation was absent in sub-cutaneous capsules transplanted in SCID mice.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Trans-differentiation ; Osteoarthritis ; Cartilage Regeneration ; Cellular Reprogramming; Pluripotent Stem-cells; Chondrocyte Differentiation; Direct Conversion; Chondrogenesis; Expression; Mouse; Cardiomyocytes; Pathway
ISSN (print) / ISBN 2073-4409
e-ISSN 2073-4409
Zeitschrift Cells
Quellenangaben Band: 9, Heft: 1, Seiten: , Artikelnummer: 191 Supplement: ,
Verlag MDPI
Verlagsort Basel