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Multicolor labeling and tracing of pancreatic beta-cell proliferation in zebrafish.
In: Animal Models of Diabetes. Berlin [u.a.]: Springer, 2020. 159-179 (Methods Mol. Biol. ; 2128)
During embryogenesis, beta-cells arise from the dorsal and ventral bud originating in the endoderm germ layer. As the animal develops to adulthood, the beta-cell mass dramatically increases. The expansion of the beta-cell population is driven by cell division among the embryonic beta-cells and supplanted by neogenesis from post-embryonic progenitors. Here, we describe a protocol for multicolor clonal analysis in zebrafish to define the contribution of individual embryonic beta-cells to the increase in cell numbers. This technique provides insights into the proliferative history of individual beta-cells in an islet. This insight helps in defining the replicative heterogeneity among individual beta-cells during development. Additionally, the ability to discriminate individual cells based on unique color signatures helps quantify the volume occupied by beta-cells and define the contribution of cellular size to the beta-cell mass.
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Publication type Article: Edited volume or book chapter
Keywords Brainbow ; Heterogeneity ; Lineage Tracing ; Quiescence ; Single Cell
ISSN (print) / ISBN 1064-3745
Book Volume Title Animal Models of Diabetes
Journal Methods in Molecular Biology
Quellenangaben Volume: 2128, Pages: 159-179
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed
Institute(s) Institute for Pancreatic Beta Cell Research (IPI)