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Cappellini, F.* ; Di Bucchianico, S. ; Karri, V.* ; Latvala, S.* ; Malmlöf, M.* ; Kippler, M.* ; Elihn, K.* ; Hedberg, J.* ; Wallinder, I.O.* ; Gerde, P.* ; Karlsson, H.L.*

Dry generation of CeO2 nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.

Nanomaterials 10:618 (2020)
Verlagsversion DOI
Open Access Gold
Creative Commons Lizenzvertrag
Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO2 NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1 beta release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO2 NPs was generated by using the PreciseInhale (R) system, and NPs were deposited on the co-culture using XposeALI (R). No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1 beta, IL-6, TNF alpha, MCP-1) were observed after exposure of the co-culture in ALI (max 5 mu g/cm(2)) or submerged (max 22 mu g/cm(2)) conditions. In contrast, CeO2 NPs cause clear IL-1 beta release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Nanotoxicology ; Air-liquid Interface ; Preciseinhale ; Dosimetry ; Inflammation ; Ceria; In-vitro; Toxicity; Aerosols; Size
ISSN (print) / ISBN 2079-4991
e-ISSN 2079-4991
Zeitschrift Nanomaterials
Quellenangaben Band: 10, Heft: 4, Seiten: , Artikelnummer: 618 Supplement: ,
Verlag MDPI
Verlagsort Basel
Begutachtungsstatus Peer reviewed