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Hindelang, B. ; Aguirre Bueno, J. ; Berezhnoi, A. ; He, H. ; Eyerich, K.* ; Ntziachristos, V. ; Biedermann, T.* ; Darsow, U.*

Optoacoustic mesoscopy shows potential to increase accuracy of allergy patch testing.

Contact Dermatitis 83, 206-214 (2020)
Postprint DOI
Open Access Green
Background Differentiation between irritant and allergic skin reactions in epicutaneous patch testing is based largely on subjective clinical criteria, with the risk of high intraobserver and interobserver variability. Novel dermatological imaging using optoacoustic mesoscopy allows quantitative three-dimensional assessment of microvascular biomarkers.Objectives We investigated the potential of optoacoustic imaging to improve the precision of patch test evaluation.Methods Sixty-nine test reactions and 48 healthy skin sections in 52 patients with suspected type IV allergy were examined using raster-scan optoacoustic mesoscopy.Results We identified biomarkers from the optoacoustic images. Allergic reactions were associated with higher fragmentation of skin vasculature than irritant reactions (19.5 +/- 9.7 vs 14.3 +/- 3.7 fragments/100 pixels(2); P < .05), as well as lower ratio of low- to high-frequency acoustic signals (1.6 +/- 0.5 vs 2.0 +/- 0.6, P < .05). Allergic reactions graded "++" showed higher vessel fragmentation than reactions graded "+" (25.4 +/- 13.2 vs 17.1 +/- 6.5 fragments/100 pixels(2); P < .05). A linear model combining the biomarkers fragmentation and frequency ratio could differentiate allergic from irritant test reactions with an area under the receiving operator characteristic curve of 0.80 (95% confidence interval 0.64-0.91), reaching a sensitivity of 81% and specificity of 63%.Conclusions Optoacoustic mesoscopy shows potential to help in differentiating between allergic and irritant test reactions based on novel biomarkers that may reflect vasodilation, vessel tortuosity, and edema.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Allergy ; Contact Dermatitis ; Optoacoustic Imaging ; Patch Test ; Photoacoustic Imaging; Optical Coherence Tomography; Irritant Contact-dermatitis; Noninvasive Evaluation; Quantification; Ultrasound; Microscopy; Nickel
ISSN (print) / ISBN 0105-1873
e-ISSN 1600-0536
Zeitschrift Contact Dermatitis
Quellenangaben Band: 83, Heft: 3, Seiten: 206-214 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort 111 River St, Hoboken 07030-5774, Nj Usa
Begutachtungsstatus Peer reviewed
Förderungen Deutsche Forschungsgemeinschaft (DFG)
European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme
European Union's Horizon 2020 research and innovation programme