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Generation of an INSULIN-H2B-Cherry reporter human iPSC line.

Stem Cell Res. 45:101797 (2020)
Verlagsversion Postprint Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Differentiating human induced pluripotent stem cells (hiPSCs) into insulin (INS)-producing beta-like cells has potential for diabetes research and therapy. Here, we generated a heterozygous fluorescent hiPSC reporter, labeling INS-producing beta-like cells. We used CRISPR/Cas9 technology to knock-in a T2A-H2B-Cherry cassette to replace the translational INS stop codon, enabling co-transcription and T2A-peptide mediated co-translational cleavage of INS-T2A and H2B-Cherry. The hiPSC-INS-T2A-H2B-Cherry reporter cells were pluripotent and showed multi-lineage differentiation potential. Cells expressing the beta-cell specific hormone INS are identified by nuclear localized H2B-Cherry reporter upon pancreatic endocrine differentiation. Thus, the generated reporter hiPSCs enable live identification of INS hormone-producing beta-like cells.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
ISSN (print) / ISBN 1873-5061
e-ISSN 1876-7753
Zeitschrift Stem Cell Research
Quellenangaben Band: 45, Heft: , Seiten: , Artikelnummer: 101797 Supplement: ,
Verlag Elsevier
Verlagsort Radarweg 29, 1043 Nx Amsterdam, Netherlands
Begutachtungsstatus Peer reviewed