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Chemical-genomic dissection of the CTD code.
Nat. Struct. Mol. Biol. 17, 1154-1161 (2010)
Sequential modifications of the RNA polymerase II (Pol II) C-terminal domain (CTD) coordinate the stage-specific association and release of cellular machines during transcription. Here we examine the genome-wide distributions of the 'early' (phospho-Ser5 (Ser5-P)), 'mid' (Ser7-P) and 'late' (Ser2-P) CTD marks. We identify gene class-specific patterns and find widespread co-occurrence of the CTD marks. Contrary to its role in 3'-processing of noncoding RNA, the Ser7-P marks are placed early and retained until transcription termination at all Pol II-dependent genes. Chemical-genomic analysis reveals that the promoter-distal Ser7-P marks are not remnants of early phosphorylation but are placed anew by the CTD kinase Bur1. Consistent with the ability of Bur1 to facilitate transcription elongation and suppress cryptic transcription, high levels of Ser7-P are observed at highly transcribed genes. We propose that Ser7-P could facilitate elongation and suppress cryptic transcription.
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Publication type Article: Journal article
Document type Scientific Article
Keywords RNA-polymerase-II; Cryptic unstable transcripts; C-terminal domain; P-TEFB; Saccaromyces-cerevisiae; Bidirectional promoters; Histone modification; Basal transcription; Processing factors; Eukaryotic genome
ISSN (print) / ISBN 1545-9993
Quellenangaben Volume: 17, Issue: 9, Pages: 1154-1161
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed