PuSH - Publication Server of Helmholtz Zentrum München

In situ metabolite mass spectrometry imaging: New insights into the adrenal gland.

Horm. Metab. Res. 52, 435-447 (2020)
Postprint DOI
Open Access Green
The adrenal gland integrates catecholamine-producing neuroendocrine cells and steroid-producing cells with mesenchymal origin in a structured manner under one capsule and is a key regulator for vital bioactivity. In addition to adrenal-specific disease, dysregulation of adrenal hormones is associated with systemic effects, leading to undesirable metabolic and cardiovascular consequences. Mass spectrometry imaging (MSI) technique can simultaneously measure a broad range of biomolecules, including metabolites and hormones, which has enabled the study of tissue metabolic and hormone alterations in adrenal and adrenal-related diseases. Furthermore, this technique coupled with labeled immunohistochemistry staining has enabled the study of the pathophysiological adaptation of the adrenal gland under normal and abnormal conditions at different molecular levels. This review discusses the recent applications of in situ MSI in the adrenal gland. For example, the combination of formalin-fixed paraffin-embedded tissue microarray and MSI to tissues from patient cohorts has facilitated the discovery of clinically relevant prognostic biomolecules and generated promising hypotheses for new sights into physiology and pathophysiology of adrenal gland. MSI also has enabled the discovery of clinically significant tissue molecular (i. e., biomarker) and pathway changes in adrenal disease, particularly in adrenal tumors. In addition, MSI has advanced the ability to optimally identify and detect adrenal gland specific molecules. Thus, as a novel analytical methodology, MSI has provided unprecedented capabilities for in situ tissue study.
Altmetric
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Review
Keywords Mass Spectrometry Imaging ; Adrenal Gland ; Steroid Hormones ; Catecholamines ; Metabolite Imaging; Tissue; Cancer; Metabolomics; Metabolites; Sections; Situ; 18-oxocortisol; Identification; Localization; Aldosterone
ISSN (print) / ISBN 0018-5043
e-ISSN 1439-4286
Quellenangaben Volume: 52, Issue: 6, Pages: 435-447 Article Number: , Supplement: ,
Publisher Thieme
Publishing Place Rudigerstr 14, D-70469 Stuttgart, Germany
Reviewing status Peer reviewed