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Burton, A. ; Brochard, V.* ; Galan, C.* ; Ruiz-Morales, E.R.* ; Rovira, Q.* ; Rodriguez-Terrones, D.* ; Kruse, K.* ; Le Gras, S.* ; Udayakumar, V.S.* ; Chin, H.G.* ; Eid, A. ; Liu, X.* ; Wang, C.* ; Gao, S.* ; Pradhan, S.* ; Vaquerizas, J.M.* ; Beaujean, N.* ; Jenuwein, T.* ; Torres-Padilla, M.E.

Heterochromatin establishment during early mammalian development is regulated by pericentromeric RNA and characterized by non-repressive H3K9me3.

Nat. Cell Biol. 22, 767-778 (2020)
Verlagsversion Postprint DOI
Open Access Green
Following fertilization in mammals, the gametes are reprogrammed to create a totipotent zygote, a process that involves de novo establishment of chromatin domains. A major feature occurring during preimplantation development is the dramatic remodelling of constitutive heterochromatin, although the functional relevance of this is unknown. Here, we show that heterochromatin establishment relies on the stepwise expression and regulated activity of SUV39H enzymes. Enforcing precocious acquisition of constitutive heterochromatin results in compromised development and epigenetic reprogramming, which demonstrates that heterochromatin remodelling is essential for natural reprogramming at fertilization. We find that de novo H3K9 trimethylation (H3K9me3) in the paternal pronucleus after fertilization is catalysed by SUV39H2 and that pericentromeric RNAs inhibit SUV39H2 activity and reduce H3K9me3. De novo H3K9me3 is initially non-repressive for gene expression, but instead bookmarks promoters for compaction. Overall, we uncover the functional importance for the restricted transmission of constitutive heterochromatin during reprogramming and a non-repressive role for H3K9me3.Burton et al. show that H3K9me3 deposition catalysed by SUV39H2 and regulated by pericentromeric RNAs in the mouse paternal pronucleus does not suppress gene expression, but bookmarks promoters for compaction.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter H3 Lysine-9 Methylation; Histone Modification; Nuclear Transfer; Dna Methylation; Gene-expression; Read Alignment; Cloned Embryos; Stem-cells; Chromatin; Seq
ISSN (print) / ISBN 1465-7392
e-ISSN 1476-4679
Zeitschrift Nature Cell Biology
Quellenangaben Band: 22, Heft: 7, Seiten: 767-778 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort Macmillan Building, 4 Crinan St, London N1 9xw, England
Begutachtungsstatus Peer reviewed