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Kulich, I.* ; Vogler, F.* ; Bleckmann, A.* ; Cyprys, P.* ; Lindemeier, M.* ; Fuchs, I.* ; Krassini, L.* ; Schubert, T.* ; Steinbrenner, J.* ; Beynon, J.* ; Falter-Braun, P. ; Längst, G.* ; Dresselhaus, T.* ; Sprunck, S.*

ARMADILLO REPEAT ONLY proteins confine Rho GTPase signalling to polar growth sites.

Nat. Plants 6, 1275–1288 (2020)
Open Access Green as soon as Postprint is submitted to ZB.
Polar growth requires the precise tuning of Rho GTPase signalling at distinct plasma membrane domains. The activity of Rho of plant (ROP) GTPases is regulated by the opposing action of guanine nucleotide-exchange factors (GEFs) and GTPase-activating proteins (GAPs). Whereas plant-specific ROPGEFs have been shown to be embedded in higher-level regulatory mechanisms involving membrane-bound receptor-like kinases, the regulation of GAPs has remained enigmatic. Here, we show that threeArabidopsisARMADILLO REPEAT ONLY (ARO) proteins are essential for the stabilization of growth sites in root hair cells and trichomes. AROs interact with ROP1 enhancer GAPs (RENGAPs) and bind to the plasma membrane via a conserved polybasic region at the ARO amino terminus. The ectopic spreading of ROP2 inaro2/3/4mutant root hair cells and the preferential interaction of AROs with active ROPs and anionic phospholipids suggests that AROs recruit RENGAPs into complexes with ROPs to confine ROP signalling to distinct membrane regions.Cell polarity that requires asymmetrical distribution of cellular components is important for plant growth and development. Here the authors identify a group of ARMADILLO domain proteins that collectively act in the polarized cell expansion of tip-growing cells by recruiting Rho of plant 1 (ROP1) enhancer GTPase-activating proteins and therefore ROP signalling to distinct plasma membrane sites.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Pollen Germination; Cell Polarity; Rop Gtpases; Tip Growth; Arabidopsis; Family; Regulator; Cloning; Transcriptome; Organization
ISSN (print) / ISBN 2055-026X
e-ISSN 2055-0278
Journal Nature Plants
Quellenangaben Volume: 6, Issue: 10, Pages: 1275–1288 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Network Biology (INET)
Grants German Research Foundation
Alexander von Humboldt Foundation