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Santovito, D.* ; Egea, V.* ; Bidzhekov, K.* ; Natarelli, L.* ; Mourao, A. ; Blanchet, X.* ; Wichapong, K.* ; Aslani, M.* ; Brunßen, C.* ; Horckmans, M.* ; Hristov, M.* ; Geerlof, A. ; Lutgens, E.* ; Daemen, M.J.A.P.* ; Hackeng, T.* ; Ries, C.* ; Chavakis, T.* ; Morawietz, H.* ; Naumann, R.* ; Hundelshausen, P.V.* ; Steffens, S.* ; Duchêne, J.* ; Megens, R.T.A.* ; Sattler, M. ; Weber, C.*

Autophagy unleashes noncanonical microRNA functions.

Autophagy 16, 2294-2296 (2020)
DOI Verlagsversion bestellen
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression which act by guiding AGO (argonaute) proteins to target RNA transcripts in the RNA-induced silencing complex (RISC). This macromolecular complex includes multiple additional components (e.g., TNRC6A) that allow for interaction with enzymes mediating inhibition of translation or RNA decay. However, miRNAs also reside in low-molecular weight complexes without being engaged in target repression, and their function in this context is largely unknown. Our recent findings show that endothelial cells exposed to protective high-shear stress or MTORC inhibition activate the macroautophagy/autophagy machinery to sustain viability by promoting differential trafficking ofMIR126strands and by enabling unconventional features ofMIR126-5p. WhereasMIR126-3pis degraded upon autophagy activation,MIR126-5pinteracts with the RNA-binding protein MEX3A to form a ternary complex with AGO2. This complex forms on the autophagosomal surface and facilitates its nuclear localization. Once in the nucleus,MIR126-5pdissociates from AGO2 and establishes aptamer-like interactions with the effector CASP3 (caspase 3). The binding toMIR126-5pprevents dimerization and proper active site formation of CASP3, thus inhibiting proteolytic activity and limiting apoptosis. Disrupting this pathwayin vivoby genetic deletion ofMex3aor by specific deficiency of endothelial autophagy aggravates endothelial apoptosis and exacerbates the progression of atherosclerosis. The direct inhibition of CASP3 byMIR126-5preveals a non-canonical mechanism by which miRNAs can modulate protein function and mediate the autophagy-apoptosis crosstalk.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Microrna ; Atherosclerosis ; Autophagy ; Mex3a ; Mir-126-5p ; Noncanonical Mirna Functions ; Endothelial Cells
ISSN (print) / ISBN 1554-8627
e-ISSN 1554-8635
Zeitschrift Autophagy
Quellenangaben Band: 16, Heft: 12, Seiten: 2294-2296 Artikelnummer: , Supplement: ,
Verlag Landes Bioscience
Verlagsort 530 Walnut Street, Ste 850, Philadelphia, Pa 19106 Usa
Begutachtungsstatus Peer reviewed
Förderungen European Research Council: [ERC]
Deutsche Forschungsgemeinschaft (DFG)