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Szabo, Q.* ; Donjon, A.* ; Jerković, I.* ; Papadopoulos, G.L.* ; Cheutin, T.* ; Bonev, B. ; Nora, E.P.* ; Bruneau, B.G.* ; Bantignies, F.* ; Cavalli, G.*

Regulation of single-cell genome organization into TADs and chromatin nanodomains.

Nat. Genet. 52, 1151-1157 (2020)
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Super-resolution microscopy identifies sub-topologically associating domain (TAD) nanodomains and intercellular heterogeneity in TAD conformation and insulation. Cohesin or CTCF depletion regulates distinct types of chromatin contacts at the TAD but not nanodomain level.The genome folds into a hierarchy of three-dimensional structures within the nucleus. At the sub-megabase scale, chromosomes form topologically associating domains (TADs)(1-4). However, how TADs fold in single cells is elusive. Here, we reveal TAD features inaccessible to cell population analysis by using super-resolution microscopy. TAD structures and physical insulation associated with their borders are variable between individual cells, yet chromatin intermingling is enriched within TADs compared to adjacent TADs in most cells. The spatial segregation of TADs is further exacerbated during cell differentiation. Favored interactions within TADs are regulated by cohesin and CTCF through distinct mechanisms: cohesin generates chromatin contacts and intermingling while CTCF prevents inter-TAD contacts. Furthermore, TADs are subdivided into discrete nanodomains, which persist in cells depleted of CTCF or cohesin, whereas disruption of nucleosome contacts alters their structural organization. Altogether, these results provide a physical basis for the folding of individual chromosomes at the nanoscale.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cohesin; Domains; Insulation; Generation; Microscopy; Principles; Landscape; Provides; Design; Scale
ISSN (print) / ISBN 1061-4036
e-ISSN 1546-1718
Zeitschrift Nature Genetics
Quellenangaben Band: 52, Heft: 11, Seiten: 1151-1157 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
Förderungen Research and La Ligue Nationale Contre le Cancer
European Research Council
European Molecular Biology Organization Long-Term Fellowship
Laboratory of Excellence EpiGenMed
Agence Nationale de la Recherche
Sir Henry Wellcome Postdoctoral Fellowship
National Institutes of Health/National Heart, Lung, and Blood Institute
European Union's Horizon 2020 research and innovation programme (MuG)
Fondation pour la Recherche Medicale
MSDAVENIR foundation (Project GENE-IGH)
French National Cancer Institute (INCa Project)
French Ministry of Higher Education and Research
La Ligue Nationale Contre le Cancer