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Hoefner, L.* ; Luther, A.* ; Palladini, A. ; Froehlich, T.* ; Waberski, D.*

Tolerance of stored boar spermatozoa to autologous seminal plasma: A proteomic and lipidomic approach.

Int. J. Mol. Sci. 21:6474 (2020)
Verlagsversion Forschungsdaten DOI
Open Access Gold
Creative Commons Lizenzvertrag
Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n= 4-5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n= 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p< 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Boar Seminal Plasma ; Sperm Quality ; Semen Preservation ; Biomarker ; Proteomics ; Lipidomics; Quantitative-analysis; High-throughput; Sperm; Identification; Proteins; Spermadhesins; Cholesterol; Granulins; Motility; Cloning
ISSN (print) / ISBN 1422-0067
e-ISSN 1661-6596
Quellenangaben Band: 21, Heft: 18, Seiten: , Artikelnummer: 6474 Supplement: ,
Verlag MDPI
Verlagsort Basel
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Pancreatic Beta Cell Research (IPI)
Förderungen German Centre for Diabetes Research (DZD e.V.)
Association for Bioeconomy Research (FBF e.V.)