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Monteagudo-Sánchez, A.* ; Hernandez Mora, J.R.* ; Simon, C.* ; Burton, A. ; Tenorio, J.* ; Lapunzina, P.* ; Clark, S.* ; Esteller, M.* ; Kelsey, G.* ; López-Siguero, J.P.* ; de Nanclares, G.P.* ; Torres-Padilla, M.E. ; Monk, D.*

The role of ZFP57 and additional KRAB-zinc finger proteins in the maintenance of human imprinted methylation and multi-locus imprinting disturbances.

Nucleic Acids Res. 48, 11394-11407 (2020)
Publ. Version/Full Text Research data DOI
Open Access Gold
Creative Commons Lizenzvertrag
Genomic imprinting is an epigenetic process regulated by germline-derived DNA methylation that is resistant to embryonic reprogramming, resulting in parental origin-specific monoallelic gene expression. A subset of individuals affected by imprinting disorders (IDs) displays multi-locus imprinting disturbances (MLID), which may result from aberrant establishment of imprinted differentially methylated regions (DMRs) in gametes or their maintenance in early embryogenesis. Here we investigated the extent of MLID in a family harbouring a ZFP57 truncating variant and characterize the interactions between human ZFP57 and the KAP1 co-repressor complex. By ectopically targeting ZFP57 to reprogrammed loci in mouse embryos using a dCas9 approach, we confirm that ZFP57 recruitment is sufficient to protect oocyte-derived methylation from reprogramming. Expression profiling in human pre-implantation embryos and oocytes reveals that unlike in mice, ZFP57 is only expressed following embryonic-genome activation, implying that other KRAB-zinc finger proteins (KZNFs) recruit KAP1 prior to blastocyst formation. Furthermore, we uncover ZNF202 and ZNF445 as additional KZNFs likely to recruit KAP1 to imprinted loci during reprogramming in the absence of ZFP57. Together, these data confirm the perplexing link between KZFPs and imprint maintenance and highlight the differences between mouse and humans in this respect.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Dna Methylation; Analysis Reveals; Gene; Hypomethylation; Mechanisms; Mutations; Chromatin; Isoforms; Loci
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Quellenangaben Volume: 48, Issue: 20, Pages: 11394-11407 Article Number: , Supplement: ,
Publisher Oxford University Press
Publishing Place Great Clarendon St, Oxford Ox2 6dp, England
Reviewing status Peer reviewed
Grants Internal UEA funding
European Union Regional Development Fund (FEDER)
Instituto de Salud Carlos III (Institute of Health Carlos III) of the Spanish Ministry of Economy and Competitiveness
European Regional Development Fund
Department of Health of the Basque Government
National Agency of Research (ISCIII-FEDER)
UK Medical Research Council (MRC)
Biotechnology and Biological Sciences Research Council (BBSRC)
FPI PhD studentship from MINECO
EMBO short-term fellowship
Spanish Ministry of Economy and Competitiveness (MINECO)