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Doryab, A. ; Taskin, M.B.* ; Stahlhut, P.* ; Schröppel, A. ; Wagner, D.E.* ; Groll, J.* ; Schmid, O.

A biomimetic, copolymeric membrane for cell-stretch experiments with pulmonary epithelial cells at the air-liquid interface.

Adv. Func. Mat., DOI: 10.1002/adfm.202004707 (2020)
Publ. Version/Full Text DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(epsilon-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (0.5 mu m) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air-liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell-stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F-actin cytoskeleton filaments and tight junctions while non-physiologic over-stretch induces cell apoptosis, activates inflammatory response (IL-8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Alveolar‐ ; Capillary Barrier ; Cyclic Mechanical Stretch ; Hybrid Polymers ; In Vitro Cell‐ ; Stretch Model ; Tunable Ultra‐ ; Thin Biphasic Membrane; Tight Junction; Nanoparticles; Permeability; Absorption; Delivery; Scaffold; Science; Barrier; Blood
ISSN (print) / ISBN 1616-301X
e-ISSN 1616-3028
Publisher Wiley
Publishing Place Weinheim
Reviewing status Peer reviewed
Grants Projekt DEAL