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Generation of a heterozygous C-peptide-mCherry reporter human iPSC line (HMGUi001-A-8).

Stem Cell Res. 50:102126 (2021)
Publ. Version/Full Text DOI
Open Access Gold
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The peptide hormone insulin produced by pancreatic β-cells undergoes post-transcriptional processing before secretion. In particular, C-peptide is cleaved from pro-insulin to generate mature insulin. Here, we introduce a C-peptide-mCherry human iPSC line (HMGUi001-A-8). The line was generated by CRISPR/Cas9 mediated heterozygous insertion of the mCherry sequence into exon 3 of the insulin locus. We demonstrate that the line is pluripotent and efficiently differentiates towards pancreatic β-like cells, which localize a red fluorescent C-peptide-mCherry fusion protein in insulin containing granules. Hence, the HMGUi001-A-8 line is a valuable resource to purify derived β-like cells and follow insulin-containing granules in real time.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 1873-5061
e-ISSN 1876-7753
Quellenangaben Volume: 50, Issue: , Pages: , Article Number: 102126 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Reviewing status Peer reviewed
Grants HumEN consortium - European Union's Seventh Framework Programme for Research, Technological Development and Demonstration
Deutsches Zentrum fur Diabetesforschung (DZD)
Helmholtz-Gemeinschaft (Helmholtz Portfolio Theme 'Metabolic Dysfunction and Common Disease)