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Kabiri, Y.* ; von Toerne, C. ; Fontes, A. ; Knolle, P.A.* ; Zischka, H.

Isolation and purification of mitochondria from cell culture for proteomic analyses.

In: Proteomic Profiling. Berlin [u.a.]: Springer, 2021. 411-419 (Methods Mol. Biol. ; 2261)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
In-depth analysis of the mitochondrial proteome can be greatly improved by analyzing isolated mitochondria instead of whole cells. However, isolation of sufficient amounts of mitochondria from cell culture has proven to be notoriously difficult due to small sample size. Thus, we have developed a reproducible, controllable, and highly customizable method to isolate high microgram to low milligram amounts of intact mitochondria from cell culture samples along with an optional density gradient purification. This chapter provides a methodological update of our approach and underlines the excellent quality and coverage of the mitochondrial proteome of crude and purified mitochondria from cultured liver cancer cell lines.
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Publication type Article: Edited volume or book chapter
Keywords Balch Homogenizer ; Cell Culture ; Mitochondria ; Proteomics
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Book Volume Title Proteomic Profiling
Quellenangaben Volume: 2261, Issue: , Pages: 411-419 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed