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Zhu, H.* ; Blahnová, V.H.* ; Perale,G.* ; Xiao, J.* ; Betge, F.* ; Boniolo, F ; Filová, E.* ; Lyngstadaas, S.B.* ; Haugen, H.J.*

Xeno-hybrid bone graft releasing biomimetic proteins promotes osteogenic differentiation of hMSCs.

Front. Cell Dev. Biol. 8:619111 (2020)
Publ. Version/Full Text Research data DOI
Open Access Gold
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Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep®, with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep® has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Bioactive Proteins ; Bone Graft ; Bone Regeneration Biomimetic ; Bone Scaffold ; Intrinsically Disordered ; Mesenchymal Stem Cells ; Xenograft; Anorganic Bovine Bone; Morphogenetic Protein-2; Mechanical-properties; Synthetic Peptides; Substitutes; Biomaterials; Scaffolds; Growth; Defect; Amelogenin
ISSN (print) / ISBN 2296-634X
e-ISSN 2296-634X
Quellenangaben Volume: 8, Issue: , Pages: , Article Number: 619111 Supplement: ,
Publisher Frontiers
Publishing Place Lausanne
Reviewing status Peer reviewed
Grants EU Horizon 2020 MSCARISE-2018-Research and Innovation Staff Exchange programme project IP Osteo "Induced pluripotent stem cell for bone and cartilage defects"
China Scholarship Council
National Key Research and Development Program of China
Eureka Eurostars Project