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Isolation of proteins from extracellular vesicles (EVs) for mass spectrometry-based proteomic analyses.

In: Proteomic Profiling. Berlin [u.a.]: Springer, 2021. 207-212 (Methods Mol. Biol. ; 2261)
Open Access Green as soon as Postprint is submitted to ZB.
Extracellular vesicles (EVs) are freely circulating nano/micrometer-sized membrane-bound vesicles released by various cell types. Their cargo consists of proteins, lipids, metabolites, and different types of RNA molecules reflecting the origin of the secreting cell type or tissue. Since the EV cargo is constantly changing in response to pathological status or different environmental stressors, it has been extensively studied in the quest for biomarkers, especially in the cancer research. Mass spectrometry (MS)-based proteome analysis is a powerful tool to elucidate the protein cargo in EVs. This chapter describes and characterizes three MS-compatible lysis methods, namely by using urea, guanidium hydrochloride, and radioimmunoprecipitation buffer for isolating proteins from EVs.
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Publication type Article: Edited volume or book chapter
Keywords Data-dependent Acquisition ; Exosomes ; Extracellular Vesicles (evs) ; Mass Spectrometry ; Proteomics ; Sample Preparation
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Book Volume Title Proteomic Profiling
Quellenangaben Volume: 2261, Issue: , Pages: 207-212 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed