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Ferreira, N.d.S.* ; Sant' Anna, F.H.* ; Reis, V.M.* ; Ambrosini, A.* ; Volpiano, C.G.* ; Rothballer, M. ; Schwab, S.* ; Baura, V.A.* ; Balsanelli, E.* ; Pedrosa, F.d.O.* ; Pereira Passaglia, L.M.* ; de Souza, E.M.* ; Hartmann, A.* ; Cassan, F.D.* ; Zilli, J.E.*

Genome-based reclassification of Azospirillum brasilense Sp245 as the type strain of Azospirillum baldaniorum sp. nov.

Int. J. Syst. Evol. Microbiol. 70, 6203-6212 (2020)
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Azospirillum sp. strain Sp245(T), originally identified as belonging to Azospirillum brasilense, is recognized as a plant-growth-promoting rhizobacterium due to its ability to fix atmospheric nitrogen and to produce plant-beneficial compounds. Azospirillum sp. Sp245(T) and other related strains were isolated from the root surfaces of different plants in Brazil. Cells are Gram-negative, curved or slightly curved rods, and motile with polar and lateral flagella. Their growth temperature varies between 20 to 38 degrees C and their carbon source utilization is similar to other Azospirillum species. A preliminary 16S rRNA sequence analysis showed that the new species is closely related to A. brasilense Sp7(T) and A. formosense CC-Nfb-7(T). Housekeeping genes revealed that Azospirillum sp. Sp245T, BR 12001 and Vi22 form a separate cluster from strain A. formosense CC-Nfb-7(T), and a group of strains closely related to A. brasilense Sp7(T). Overall genome relatedness index (OGRI) analyses estimated based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) between Azospirillum sp. Sp245(T) and its close relatives to other Azospirillum species type strains, such as A. brasilense Sp7(T) and A. formosense CC-Nfb-7(T) , revealed values lower than the limit of species circumscription. Moreover, core-proteome phylogeny including 1079 common shared proteins showed the independent clusterization of A. brasilense Sp7(T), A. formosense CC-Nfb-7(T) and Azospirillum sp. Sp245(T), a finding that was corroborated by the genome clustering of OGRI values and housekeeping phylogenies. The DNA G+C content of the cluster of Sp245(T) was 68.4-68.6 %. Based on the phylogenetic, genomic, phenotypical and physiological analysis, we propose that strain Sp245(T) together with the strains Vi22 and BR12001 represent a novel species of the genus Azospirillum, for which the name Azospirillum baldaniorum sp. nov. is proposed. The type strain is Sp245(T) (=BR 11005(T)=IBPPM 219(T)) (GCF_007827915.1, GCF_000237365.1, and GCF_003119195.2).
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Publication type Article: Journal article
Document type Scientific Article
Keywords Genomic Metrics ; Azospirillum ; Azospirillum Brasilense Sp7 ; Proteoma- Core Phylogeny ; Housekeeping Phylogenies; Spirillum; Algorithm; Taxonomy; Quality; Roots; Wheat
ISSN (print) / ISBN 1466-5026
e-ISSN 1466-5034
Quellenangaben Volume: 70, Issue: 12, Pages: 6203-6212 Article Number: , Supplement: ,
Publisher SGM
Publishing Place Reading
Reviewing status Peer reviewed
Institute(s) Institute of Network Biology (INET)
Grants CAPES (Coordination of Superior Level Staff Improvement)
CNPq (Brazilian National Council for Scientific and Technological Development)
Embrapa (Brazilian Agricultural Research Corporation)