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Becker, M.* ; Strengert, M.* ; Junker, D.* ; Kaiser, P.D.* ; Kerrinnes, T.* ; Traenkle, B.* ; Dinter, H.* ; Häring, J.* ; Ghozzi, S.* ; Zeck, A.* ; Weise, F.* ; Peter, A. ; Hörber, S. ; Fink, S.* ; Ruoff, F.* ; Dulovic, A.* ; Bakchoul, T.* ; Baillot, A.* ; Lohse, S.* ; Cornberg, M.* ; Illig, T.* ; Gottlieb, J.* ; Smola, S.* ; Karch, A.* ; Berger, K.* ; Rammensee, H.G.* ; Schenke-Layland, K.* ; Nelde, A.* ; Märklin, M.* ; Heitmann, J.S.* ; Walz, J.S.* ; Templin, M.* ; Joos, T.O.* ; Rothbauer, U.* ; Krause, G.* ; Schneiderhan-Marra, N.*

Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity.

Nat. Commun. 12:1152 (2021)
Publ. Version/Full Text DOI
Open Access Gold
Creative Commons Lizenzvertrag
The humoral immune response to SARS-CoV-2 is a benchmark for immunity and detailed analysis is required to understand the manifestation and progression of COVID-19, monitor seroconversion within the general population, and support vaccine development. The majority of currently available commercial serological assays only quantify the SARS-CoV-2 antibody response against individual antigens, limiting our understanding of the immune response. To overcome this, we have developed a multiplex immunoassay (MultiCoV-Ab) including spike and nucleocapsid proteins of SARS-CoV-2 and the endemic human coronaviruses. Compared to three broadly used commercial in vitro diagnostic tests, our MultiCoV-Ab achieves a higher sensitivity and specificity when analyzing a well-characterized sample set of SARS-CoV-2 infected and uninfected individuals. We find a high response against endemic coronaviruses in our sample set, but no consistent cross-reactive IgG response patterns against SARS-CoV-2. Here we show a robust, high-content-enabled, antigen-saving multiplex assay suited to both monitoring vaccination studies and facilitating epidemiologic screenings for humoral immunity towards pandemic and endemic coronaviruses.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Quellenangaben Volume: 12, Issue: 1, Pages: , Article Number: 1152 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Grants European Union
Initiative and Networking Fund of the Helmholtz Association of German Research Centres