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Di Giosia, M.* ; Solda, A. ; Seeger, M. ; Cantelli, A.* ; Arnesano, F.* ; Nardella, M.I.* ; Mangini, V.* ; Valle, F.* ; Montalti, M.* ; Zerbetto, F.* ; Rapino, S.* ; Calvaresi, M.* ; Ntziachristos, V.

A bio-conjugated fullerene as a subcellular-targeted and multifaceted phototheranostic agent.

Adv. Func. Mat. 31:2101527 (2021)
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Open Access Gold (Paid Option)
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Fullerenes are candidates for theranostic applications because of their high photodynamic activity and intrinsic multimodal imaging contrast. However, fullerenes suffer from low solubility in aqueous media, poor biocompatibility, cell toxicity, and a tendency to aggregate. C70@lysozyme is introduced herein as a novel bioconjugate that is harmless to a cellular environment, yet is also photoactive and has excellent optical and optoacoustic contrast for tracking cellular uptake and intracellular localization. The formation, water-solubility, photoactivity, and unperturbed structure of C70@lysozyme are confirmed using UV-visible and 2D 1H, 15N NMR spectroscopy. The excellent imaging contrast of C70@lysozyme in optoacoustic and third harmonic generation microscopy is exploited to monitor its uptake in HeLa cells and lysosomal trafficking. Last, the photoactivity of C70@lysozyme and its ability to initiate cell death by means of singlet oxygen (1O2) production upon exposure to low levels of white light irradiation is demonstrated. This study introduces C70@lysozyme and other fullerene-protein conjugates as potential candidates for theranostic applications.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Fullerenes ; Lysozymes ; Multimodal Microscopy ; Optoacoustic Imaging ; Photodynamic Therapy ; Phototheranostic Agents
ISSN (print) / ISBN 1616-301X
e-ISSN 1616-3028
Quellenangaben Volume: 31, Issue: 20, Pages: , Article Number: 2101527 Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Reviewing status Peer reviewed
Grants Italian Ministry of Education, University and Research
Gottfried Wilhelm Leibniz Prize
Deutsche Forschungsgemeinschaft (DFG)
FIRC-AIRC fellowship for Italy
Italian Ministry of Education, University and Research (MIUR), SIR Programme