PuSH - Publication Server of Helmholtz Zentrum München

Huge splicing frequency in human Y chromosomal UTY gene.

OMICS 15, 141-154 (2011)
Publ. Version/Full Text DOI
Over 90% of human genes produce more than one mRNA by alternative splicing (AS). Human UTY (ubiquitously transcribed tetratricopeptide repeat protein on the chromosome Y) has six mRNA-transcripts. UTY is subject to interdisciplinary approaches such as Y chromosomal genetics or development of leukemia immunotherapy based on UTY-specific peptides. Investigating UTY expression in a normal and leukemic setting we discovered an exceptional splicing phenomenon fostering huge transcript diversity. Transcript sequencing identified 90 novel AS-events being almost randomly combined in 284 new transcripts. We uncovered a novel system of transcript architecture and genomic organization in UTY. On a basis of a new UTY-splicing multigraph including a mathematical model we calculated the theoretical yield to exceed 1.3 billion distinct transcripts. To our knowledge, this is the greatest estimated transcript diversity by AS. On protein level we demonstrated interaction of AS-derived proteins with new interactors by yeast-two-hybrid assay. For translational research we predicted new UTY-peptide candidates for leukemia therapy development. Our study provides new insights into the complexity of human alternative splicing and its potential contribution to the transcript diversity of the transcriptome.
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Keywords nonsense-mediated decay; male-specific region; messenger-rna decay; human transcriptome; proteins; generation; isoforms; cells
ISSN (print) / ISBN 1070-2830
e-ISSN 1557-8100
Journal OMICS
Quellenangaben Volume: 15, Issue: 3, Pages: 141-154 Article Number: , Supplement: ,
Publisher Mary Ann Liebert
Publishing Place New Rochelle, USA
Reviewing status Peer reviewed
Institute(s) Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Experimental Genetics (IEG)
Institute of Bioinformatics and Systems Biology (IBIS)